Identification of proteins….
Basics of protein identification are simple: Proteins are digested and the resulting peptides are analyzed by mass spectrometers to obtain their mass data. There are three different types of MS data that can be used for database search. They are
(1) molecular weights of peptides that can be used for Peptide Mass Mapping,
(2) combination of mass data and partial amino acid sequence that can be used for Sequence Tag, and
(3) tandem mass spectrometry data (uninterpreted) that are used for MS/MS fragmentation ion search.
Source: http://msf.ucdavis.edu/ms_protein_id.html
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