DNA protocol (2)

Isolation of DNA from buccal swabs
Materials
50 mM NaOH
1M Tris.Cl, pH 6.5
70% ethanol
CYTo-Pak with Cyto-soft brush (CP-5B; Medical packaging) or Caliber-Cotton-Tipped Apllicator swab (size 6 in; Allegiance Healthcare)
95 degree heating block

DNA isolation protocol (1)

Isolation of Genomic DNA
DNA isolation from whole blood
Materials
10 ml fresh or thawed frozen whole blood
NKM buffer, 4 degree celcius
Resuspension buffer
10 X TEN solution
2 mg/ml proteinase K
10% (w/v) SDS
Buffered phenol
25:24:1 (v/v) phenol/chloroform/isoamyl alcohol
24:1 (v/v) chloroform/isoamyl alcohol
TE buffer, pH 8.0
Dailysis buffer
Refrigerated centrifuge
Orbital shaker
Dialysis tubing (MWCO 50000)
1.5 ml storage vials